Rodriguez, Heriberto
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
A recently developed triple staining (SNARF-1/YO-PRO-1/ethidium homodimer) was used to assess early changes in boar sperm membrane integrity (MI) with the results of cryopreservation procedures and to seek for correlations among MI-spermatozoa in pre-freeze semen and its freezeability. Ejaculates from five boars were evaluated in the fresh and frozen-thawed (FT) state, and its freezeability defined as % of membrane intactness, MI% (MI% = % of FT-spermatozoa with intact membranes x 100 divided by the % of prefreeze spermatozoa with intact membranes) estimated. Significant differences were found among boars for freezeability (MI%) and motility post-thaw (%). Interestingly, significant correlations were found between the percentage of YO-PRO-1-positive spermatozoa and freezeability (R = 0.440, p < 0.01), indicating this new triple staining can be used to safely disclose among ejaculates prior to freezing. (c) 2006 Elsevier B.V. All rights reserved
Publicerad i
Animal Reproduction Science
2007, volym: 97, nummer: 1-2, sidor: 74-83
Utgivare: ELSEVIER SCIENCE BV
SLU författare
Johannisson, Anders
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Veterinärmedicin
Husdjursvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.anireprosci.2005.12.014
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/16572