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Sammanfattning

Potyviral helper-component proteinase (HCpro) is a multifunctional protein exerting its cellular functions in interaction with putative host proteins. In this study, cellular protein partners of the HCpro encoded by Potato virus A (PVA) (genus Potyvirus) were screened in a potato leaf cDNA library using a yeast two-hybrid system. Two cellular proteins were obtained that interact specifically with PVA HCpro in yeast and in the two in vitro binding assays used. Both proteins are encoded by single-copy genes in the potato genome. Analysis of the deduced amino acid sequences revealed that one (HIP1) of the two HCpro interactors is a novel RING finger protein. The sequence of the other protein (HIP2) showed no resemblance to the protein sequences available from databanks and has known biological functions.

Publicerad i

Molecular plant-microbe interactions
2003, volym: 16, nummer: 5, sidor: 405-410
Utgivare: AMER PHYTOPATHOLOGICAL SOC

SLU författare

UKÄ forskningsämne

Jordbruksvetenskap

Publikationens identifierare

  • DOI: https://doi.org/10.1094/MPMI.2003.16.5.405

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/1894