Potato mop-top virus: the coat protein-encoding RNA and the gene for cysteine-rich protein are dispensable for systemic virus movement in Nicotiana benthamiana

Sammanfattning

Full-length genomic cDNA clones of the Swedish isolate of Potato mop-top virus (PMTV) were transcribed in vitro using T7 RNA polymerase. The combination of RNA 1, 2 and 3 synthesized in the presence of m(7) GpppG cap analogue was infectious when inoculated onto Nicotiana benthamiana plants. Also, the combination of RNA 1 (encodes the viral replicase) with RNA 3 [encodes the triple gene block proteins and a small cysteine-rich protein (CRP)] was infectious and both RNAs moved systemically in N. benthamiana plants in the absence of RNA 2, which encodes the coat protein (CP). However, the yellow mosaic symptoms that typically developed following PMTV infection with all three RNAs were not observed in plants infected with RNA 1 + RNA 3. Site-directed mutagenesis experiments revealed that expression of the putative CRP was not required for systemic infection and symptom induction in N. benthamiana. These data show that PMTV represents an example of a multipartite virus capable of establishing systemic infection without the CP-encoding RNA, and also without the putative CRP

Publicerad i

Journal of General Virology
2003, volym: 84, sidor: 1001-1005
Utgivare: SOC GENERAL MICROBIOLOGY

SLU författare

• Savenkov, Eugene

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Valkonen, Jari

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

• Germundsson, Anna

  • Institutionen för växtbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Jordbruksvetenskap

Publikationens identifierare

• DOI: https://doi.org/10.1099/vir.0.18813-0

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/1896