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Sammanfattning

Terminal restriction fragment length polymorphism (T-RFLP) and denaturing gradient gel electrophoresis (DGGE) and subsequent statistical analysis were compared with assess denitrifier community composition in agricultural soil based on the nosZ gene, encoding the nitrous oxide reductase. Analysis of binary or relative abundance-based metric and semi-metric distance matrices provided similar results for DGGE, but not for T-RFLP. Moreover, DGGE had a higher resolution than T-RFLP and binary data was better for discriminating between samples.

Nyckelord

agriculture; community fingerprint techniques; denitrification; microbial ecology; soil diversity

Publicerad i

Letters in Applied Microbiology
2009, volym: 48, nummer: 1, sidor: 145-148

SLU författare

UKÄ forskningsämne

Jordbruksvetenskap
Miljö- och naturvårdsvetenskap

Publikationens identifierare

  • DOI: https://doi.org/10.1111/j.1472-765X.2008.02498.x

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/20521