Wang, Liya
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
Mitochondrial thymidine kinase (TK2) is responsible for phosphorylation of thymidine and deoxycytidine and plays a crucial role in mitochondrial DNA precursor synthesis. TK2 is expressed in all tissues at low levels complicating accurate determinations, especially in tissues with high cytosolic thymidine kinase (TK1) activity. Recently, 5-bromovinyl 2'-deoxyuridine (BvdU) at 0.2 mu M was used to measure TK2 activity selectively. BvdU phosphorylation by pure human TK2 and TK1 was tested here, and the ratio of BvdU phosphorylation by TK2/TK1 was 91 at 0.2 mu M but was 500 at 2.5 mu M. Therefore, for reliable measurement of TK2 activity higher BvdU concentration should be used.
Nyckelord
thymidine kinase 2; thymidine kinase 1; activity determination; bromovinyl deoxyurindine
Publicerad i
Nucleosides, Nucleotides and Nucleic Acids
2008, volym: 27, nummer: 6-7, sidor: 858-862
Utgivare: TAYLOR & FRANCIS INC
SLU författare
Eriksson, Staffan
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Husdjursvetenskap
Veterinärmedicin
Publikationens identifierare
- DOI: https://doi.org/10.1080/15257770802146510
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/20796