Andersson, Inger
- Department of Molecular Biology, Swedish University of Agricultural Sciences
Abstract
Merohedral twinning is a crystal-growth disorder that seriously hinders the determination of macromolecular crystal structures by isomorphous replacement. The strategies used in the structures solved so far are discussed. Several methods can be used to determine the extent of twinning, the twin fraction and to detwin the data. Accurate determination of the twin fraction by analysing heavy-atom refinement statistics is possible, but only influences the resulting phases slightly. It seems more crucial to restrict the variation in twin fractions between data sets, either by making the twin fractions of some data sets artificially higher or by screening crystals to obtain data with a low twin fraction.
Keywords
MIR phasing; merohedral twinning
Published in
Acta Crystallographica Section D: Biological Crystallography
2003, volume: 59, pages: 2017-2022
Publisher: BLACKWELL MUNKSGAARD
SLU Authors
Terwisscha Van Scheltinga, Anke
- Department of Molecular Biology, Swedish University of Agricultural Sciences
- Department of Molecular Biology, Swedish University of Agricultural Sciences
Valegård, Karin
- Department of Molecular Biology, Swedish University of Agricultural Sciences
- Department of Molecular Biology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Food Science
Publication identifier
- DOI: https://doi.org/10.1107/S090744490302122X
Permanent link to this page (URI)
https://res.slu.se/id/publ/2464