MIR phasing using merohedrally twinned crystals

Sammanfattning

Merohedral twinning is a crystal-growth disorder that seriously hinders the determination of macromolecular crystal structures by isomorphous replacement. The strategies used in the structures solved so far are discussed. Several methods can be used to determine the extent of twinning, the twin fraction and to detwin the data. Accurate determination of the twin fraction by analysing heavy-atom refinement statistics is possible, but only influences the resulting phases slightly. It seems more crucial to restrict the variation in twin fractions between data sets, either by making the twin fractions of some data sets artificially higher or by screening crystals to obtain data with a low twin fraction.

Nyckelord

MIR phasing; merohedral twinning

Publicerad i

Acta Crystallographica Section D: Biological Crystallography
2003, volym: 59, sidor: 2017-2022
Utgivare: BLACKWELL MUNKSGAARD

SLU författare

• Andersson, Inger

  • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet
    

• Terwisscha Van Scheltinga, Anke

  • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet
    

• Valegård, Karin

  • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Livsmedelsvetenskap

Publikationens identifierare

• DOI: https://doi.org/10.1107/S090744490302122X

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/2464