Unell, Maria
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
We compared the Arthrobacter chlorophenolicus proteome during growth on 4-chlorophenol, 4-nitrophenol, or phenol at 5 and 28 degrees C, both for the wild-type and a mutant strain with mass spectrometry based proteomics. A label-free workflow employing spectral counting identified 3749 proteins across all growth conditions, representing over 70% of the predicted genome and 739 of these proteins form the core proteome. Statistically significant differences were found in the proteomes of cells grown under different conditions including differentiation of hundreds of unknown proteins. The 4-chlorophenol-degradation pathway was confirmed, but not that for phenol.
Nyckelord
Arthrobacter chlorophenolicus; 4-chlorophenol; 4-nitrophenol; phenol; shotgun proteomics; 2D-LC-ES-MS/MS; hydroxyquinol dioxygenase
Publicerad i
Journal of Proteome Research
2009, volym: 8, nummer: 4, sidor: 1953-1964
SLU författare
Jansson, Janet
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
- Lawrence Berkeley National Laboratory (Berkeley Lab)
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Miljö- och naturvårdsvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1021/pr800897c
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/26199