Schmitz, Monica
- Institutionen för vattenbruk, Sveriges lantbruksuniversitet
Sammanfattning
The mRNA expression of the LH-beta and FSH-beta subunits were measured in nesting and post-breeding male three-spined sticklebacks, Gasterosteus aculetaus, as well as in castrated and sham-operated nesting males. Furthermore, expression of an androgen induced kidney protein, spiggin, and 11-ketotestosterone (11KT) levels, were measured in the castrated and sham-operated males. Nesting males had significantly higher levels of both LH-beta and FSH-beta mRNA expression compared to post-breeding males. Furthermore, sham-operated males had significantly higher levels of LH-beta mRNA and spiggin mRNA expression than the castrated fish. Expression of FSH-beta, on the other hand, did not differ between castrated and sham-operated males. There were strong positive individual correlations between circulating levels of 11KT on the one hand and expressions of LH-alpha and spiggin mRNA, whereas the correlation between 11KT levels and FSH-beta mRNA was weak. The negative effect of castration on beta-LH mRNA indicates that gonadal hormones stimulate this expression, whereas this was not the case for beta-FSH. The observed decline in beta-LH expression after the end of the breeding season may be the result of cessation of the gonadal stimulation of the pituitary. On the other hand, it is not likely that this can explain the decline in FSH-beta expression.
Nyckelord
fish; FSH; gonadotropin; LH; teleost; 11-ketotestosterone
Publicerad i
Fish Physiology and Biochemistry
2001, volym: 25, nummer: 4, sidor: 311-317
Utgivare: Springer Verlag (Germany)
SLU författare
UKÄ forskningsämne
Fisk- och akvakulturforskning
Publikationens identifierare
- DOI: https://doi.org/10.1023/A:1023210407163
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/276