Selection of boar spermatozoa using centrifugation on a glycidoxypropyltrimethoxysilane-coated silica colloid

Sammanfattning

Use of sperm separation methods such as density gradient centrifugation for selecting the best spermatozoa for animal breeding is constrained by the problem of dealing with the large volumes of ejaculate produced by the males of some species, such as boars. The purpose Of this Study was to compare density gradient centrifugation (DGC) with centrifugation on a single layer of colloid (SLC) for the preparation of ejaculated boar spermatozoa using Androcoll (TM)-P. There was no difference between the two techniques in terms of sperm motility or duration of motility after selection, and sperm motility was retained for at least 24 h longer in the centrifuged sperm preparations than in controls (uncentrifuged aliquots). Sperm motility was significantly better (P

Nyckelord

Boar spermatozoa; Colloids; Density gradient centrifugation; Single layer centrifugation

Publicerad i

Journal of Reproduction and Development
2009, volym: 55, nummer: 5, sidor: 547-552

SLU författare

• Saravia-Ramos, Fernando

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Morrell, Jane

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• van Wienen, Marjet

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Wallgren, Margareta

  • Quality Genetics

• Rodriguez, Heriberto

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Veterinärmedicin
Husdjursvetenskap

Publikationens identifierare

• DOI: https://doi.org/10.1262/jrd.20243

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/27682