Jastrebova, Jelena
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Abstract
Ultra performance liquid chromatography (UPLC) using small sub-2 mu m particles and high performance liquid chromatography (HPLC) were compared for separation and determination of the most common dietary folates; 5-methyltetrahydrofolate, tetrahydrofolate, 5-formyltetrahydrofolate, 10-formylfolic acid and folic acid. Two UPLC columns-Acquity BEH C(18) and Acquity HSS T3, and two HPLC columns with similar surface chemistry-Xbridge C(18) and Atlantis d18 were tested. When using UPLC, the signal-to-noise ratio could be improved by a factor of 2-50 for different folate derivatives and the run time could be reduced fourfold without sacrificing separation efficiency. The applicability of UPLC to real food samples was demonstrated
Keywords
Ultra performance liquid chromatography; Ultra fast separation; Sub-2 mu m particles; Folate food analysis LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; HIGH-TEMPERATURE; HIGH-PRESSURE; DERIVATIVES; STABILITY; BERRIES; FINLAND; ASSAY; ACID
Published in
Chromatographia
2011, volume: 73, number: 3-4, pages: 219-225
SLU Authors
Strandler, Hanna Sara
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Patring, Johan
- Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences
UKÄ Subject classification
Food Science
Publication identifier
- DOI: https://doi.org/10.1007/s10337-010-1896-z
Permanent link to this page (URI)
https://res.slu.se/id/publ/33622