Jastrebova, Jelena
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
Ultra performance liquid chromatography (UPLC) using small sub-2 mu m particles and high performance liquid chromatography (HPLC) were compared for separation and determination of the most common dietary folates; 5-methyltetrahydrofolate, tetrahydrofolate, 5-formyltetrahydrofolate, 10-formylfolic acid and folic acid. Two UPLC columns-Acquity BEH C(18) and Acquity HSS T3, and two HPLC columns with similar surface chemistry-Xbridge C(18) and Atlantis d18 were tested. When using UPLC, the signal-to-noise ratio could be improved by a factor of 2-50 for different folate derivatives and the run time could be reduced fourfold without sacrificing separation efficiency. The applicability of UPLC to real food samples was demonstrated
Nyckelord
Ultra performance liquid chromatography; Ultra fast separation; Sub-2 mu m particles; Folate food analysis LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; HIGH-TEMPERATURE; HIGH-PRESSURE; DERIVATIVES; STABILITY; BERRIES; FINLAND; ASSAY; ACID
Publicerad i
Chromatographia
2011, volym: 73, nummer: 3-4, sidor: 219-225
SLU författare
Strandler, Hanna Sara
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
Patring, Johan
- Institutionen för vatten och miljö, Sveriges lantbruksuniversitet
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
- Institutionen för vatten och miljö, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Livsmedelsvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1007/s10337-010-1896-z
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/33622