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Sammanfattning

Two surface plasmon resonance (SPR)-based biosensor assays for detection of beta-lactam antibiotics in milk are reported. The assays are based on the enzymatic activity of a carboxypeptidase converting a 3-peptide into a 2-peptide, a reaction that is inhibited in the presence of beta-lactams. Antibodies were used to measure either the amount of formed enzymatic product or the amount of remaining enzymatic substrate. Both assays detected different beta-lactams at or below European maximum residue limits (MRLs), and the detection limit for penicillin G was 1.2 mug/kg and 1.5 mug/kg for the 2- and 3-peptide assays, respectively. The precision (CV) was < 5%, both within and between assays at the penicillin G MRL (4,mug/kg). The biosensor results obtained upon analysis of incurred milk samples were compared with results obtained by liquid chromatography (HPLC), and the method agreements were, in general, good.

Nyckelord

beta-lactams; milk; beta-lactam receptor protein; SPR biosensor

Publicerad i

Journal of Agricultural and Food Chemistry
2004, volym: 52, nummer: 10, sidor: 2791-2796
Utgivare: AMER CHEMICAL SOC

SLU författare

UKÄ forskningsämne

Livsmedelsvetenskap

Publikationens identifierare

  • DOI: https://doi.org/10.1021/jf0344284

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/3650