Andersson, Roger
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Abstract
High-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) is a widely used technique to study the chain length distribution of amylopectin. The chromatograms, however, do not directly reflect this distribution, since the PAD response changes with the degree of polymerization. In this study, waxy maize starch was debranched and fractionated on a Bio-Gel P-6 column and the response factors for maltosaccharides with DP 3-65 were determined. The detector response per mu g glucan chains was shown to decrease considerably for DP 3-7 while the curve leveled out for DP larger than 15. (C) 1998 Elsevier Science B.V.
Keywords
amylopectin; starch; saccharides
Published in
Journal of Chromatography A
1998, volume: 800, number: 2, pages: 199-206
SLU Authors
Koch, Kristine
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Åman, Per
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Food Science
Analytical Chemistry
Publication identifier
- DOI: https://doi.org/10.1016/S0021-9673(97)01151-5
Permanent link to this page (URI)
https://res.slu.se/id/publ/39610