Quantitative analysis of amylopectin unit chains by means of high-performance anion-exchange chromatography with pulsed amperometric detection

Sammanfattning

High-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) is a widely used technique to study the chain length distribution of amylopectin. The chromatograms, however, do not directly reflect this distribution, since the PAD response changes with the degree of polymerization. In this study, waxy maize starch was debranched and fractionated on a Bio-Gel P-6 column and the response factors for maltosaccharides with DP 3-65 were determined. The detector response per mu g glucan chains was shown to decrease considerably for DP 3-7 while the curve leveled out for DP larger than 15. (C) 1998 Elsevier Science B.V.

Nyckelord

amylopectin; starch; saccharides

Publicerad i

Journal of Chromatography A
1998, volym: 800, nummer: 2, sidor: 199-206

SLU författare

• Andersson, Roger

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Koch, Kristine

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Åman, Per

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Livsmedelsvetenskap
Analytisk kemi

Publikationens identifierare

• DOI: https://doi.org/10.1016/S0021-9673(97)01151-5

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/39610