Lerceteau-Köhler, Estelle
- Ciref Création Variétale fraises fruits rouges
- Karl-Franzens-Universität Graz
Sammanfattning
Bulked segregant analysis combined with AFLPs was used to identify molecular markers linked to the Rca2 gene conferring resistance to Colletotrichum acutatum pathogenicity group 2 which causes anthracnose in the octoploid strawberry Fragaria x ananassa. DNA bulks originating from a cross between the resistant cultivar 'Capitola' and the susceptible cultivar 'Pajaro' were screened with 110 EcoRI/MseI AFLP combinations. Four AFLP markers were found linked in coupling phase to Rca2 with recombination percentages between 0% and 17.7%. Among the four markers linked to the resistance gene, two were converted into SCAR markers (STS-Rca2_417 and STS-Rca2_240) and screened in a large segregating population including 179 genotypes. The Rca2 resistance gene was estimated to be 0.6 cM from STS-Rca2_417 and 2.8 cM from STS-Rca2_240. The presence/absence of the two SCAR markers was further studied in 43 cultivars of F. x ananassa, including 14 susceptible, 28 resistant, and one intermediate genotype. Results showed that 81.4% and 62.8% of the resistant/susceptible genotypes were correctly predicted by using STS-Rca2_417 and STS-Rca2_240, respectively. The 14 susceptible genotypes showed no amplification for either SCARs. These developed SCARs constitute new tools for indirect selection criteria of anthracnose resistance genotypes in strawberry breeding programs.
Publicerad i
TAG Theoretical and Applied Genetics
2005, volym: 111, nummer: 5, sidor: 862-870
Utgivare: SPRINGER
SLU författare
UKÄ forskningsämne
Genetik och genomik
Genetik och förädling inom lantbruksvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1007/s00122-005-0008-1
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/40182