Stallion sperm selection: past, present and future trends.

Sammanfattning

Although several selection techniques are available for processing spermatozoa, only colloid centrifugation has been used to any extent in this field, starting with density gradient centrifugation and progressing more recently to single-layer centrifugation (SLC). SLC through a species-specific colloid has been shown to be effective in selecting spermatozoa with good motility and normal morphology from stallion semen. The method is easier to use and less time-consuming than density gradient centrifugation, and has been scaled-up to allow whole ejaculates to be processed in a practical manner. The potential applications of SLC in equine breeding are as follows: to improve sperm quality in artificial insemination doses for "problem" ejaculates, to increase the shelf life of normal sperm doses, to remove pathogens (viruses, bacteria), to improve cryosurvival by removing dead and dying spermatozoa before freezing or after thawing, to select spermatozoa for intracytoplasmic sperm injection, and to aid conservation breeding.

Publicerad i

Journal of Equine Veterinary Science
2012, volym: 32, nummer: 8, sidor: 436-440

SLU författare

• Morrell, Jane

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Annan veterinärmedicin

Publikationens identifierare

• DOI: https://doi.org/10.1016/j.jevs.2012.05.069

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/40745