Stenlid, Jan
- Institutionen för skoglig mykologi och växtpatologi, Sveriges lantbruksuniversitet
Sammanfattning
Somatic incompatibility is not an absolute block to nuclear exchange between incompatible mycelia of the basidiomycete Heterobasidion annosum in vitro. Under laboratory conditions new heterokaryotic genotypes can be isolated from the gap between incompatible heterokaryons, and nuclear migration between pairs of heterokaryons grown in Petri dishes has been observed. In this study, we test the hypothesis of nuclear transfer and reassortment between heterokaryotic mycelia in natural populations of H. annosum. We developed six microsatellite markers to genotype nuclei populating 21 somatically incompatible mycelia of H. annosum isolated from a single stump of Picea abies, and found that the detected heterokaryons share nuclei; 10 of the nuclear haplotypes were found in more than one mycelium. In one isolate, four nuclear types were found in the same mycelium. These findings indicate that new heterokaryons can be formed as a result of nuclear reassortment between incompatible heterokaryotic mycelia in nature. (C) 2004 Elsevier Inc. All rights reserved
Nyckelord
Nuclear mosaic; Nuclear reassortment; Somatic incompatibility; Microsatellites; Root rot; Natural chimera; Fungi; Allorecognition; Individual; Operational selective unit
Publicerad i
Fungal Genetics and Biology
2004, volym: 41, nummer: 5, sidor: 563-570
Utgivare: ACADEMIC PRESS INC ELSEVIER SCIENCE
SLU författare
UKÄ forskningsämne
Miljö- och naturvårdsvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.fgb.2004.01.002
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/4106