Andersson, Kristina
- Institutionen för tillämpad husdjursvetenskap och välfärd, Sveriges lantbruksuniversitet
Sammanfattning
In the present study, we investigated hepatic mRNA expression and activities of CYP3A and 2C in entire, surgically castrated and pigs vaccinated with Improvac. Additionally, we examined the mRNA expression of the two nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR), known to regulate CYP3A and 2C mRNA expression, respectively. Activities of CYP3A and 2C were estimated as a rate of 7-benzyloxy-4-trifluoromethylcoumarin and 7-benzyloxyquinoline metabolism (CYP3A) and tolbutamide metabolism (CYP2C). We found no effect of Improvac treatment or surgical castration on either CYP3A or 2C activities. Similarly, the mRNA expressions of CYP3A29, 2C33 and PXR were not changed. CAR mRNA expression differed only between entire and surgically castrated male pigs (p = 0.005), being greater in surgically castrated pigs. Our results indicated that neither CYP3A nor 2C are affected by Improvac. (C) 2012 Elsevier Ltd. All rights reserved.
Nyckelord
Immunocastration; Tolbutamide; 7-benzyloxy-4-trifluoromethylcoumarin; 7-benzyloxyquinoline; CAR; PXR
Publicerad i
Vaccine
2012, volym: 30, nummer: 24, sidor: 3515-3518
Utgivare: ELSEVIER SCI LTD
SLU författare
Zamaratskaia, Galia
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Annan veterinärmedicin
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.vaccine.2012.03.072
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/42476