Comparison between high-resolution selected ion monitoring, selected reaction monitoring, and four-sector tandem mass spectrometry in quantitative analysis of gibberellins in milligram amounts of plant tissue

Sammanfattning

Gibberellins (GAs) were quantified in semipurified plant extracts by combined gas chromatography/mass spectrometry(GC/MS). High-resolution selected ion monitoring (HR-SIM), selected reaction monitoring (SRM), and four sector tandem mass spectrometry (MS/MS) were compared. The best selectivity was found with four-sector MS/MS analysis, but the sensitivity was too low for analysis in milligram amounts of plant tissue. HR-SIM and SRM had similarly low limits of detection, except for the analyses of GA(29), but SRM provided better selectivity. The variations of sensitivity and selectivity for the different MS methods are discussed.

Publicerad i

Analytical Chemistry
1995, volym: 67, nummer: 10, sidor: 1711-1716
Utgivare: AMER CHEMICAL SOC

SLU författare

• Moritz, Thomas

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Analytisk kemi

Publikationens identifierare

• DOI: https://doi.org/10.1021/ac00106a010

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/42908