Moritz, Thomas
- Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
Sammanfattning
Gibberellin A9 (GA9) glucosyl ester was identified in shoots of Sitka spruce [Picea sitchensis (Bong.) Carr.] grafts using capillary reversed phase high performance liquid chromatography combined on-line with a double focusing mass spectrometer. The column effluent was introduced into the ion source of the mass spectrometer via a frit-fast atom bombardment liquid chromatography/mass spectrometry interface. Negative ion mass spectra were obtained and compared with the spectrum of an authentic standard. In order to confirm the identity of the aglycone, a daughter ion spectrum from the parent ion m/z 315 was obtained by B/E linked scanning and compared with a daughter ion spectrum of free GA9. Furthermore, the elemental composition of the GA conjugate was determined by high resolution mass spectrometry.
Nyckelord
LIQUID CHROMATOGRAPHY MASS SPECTROMETRY; FAST ATOM BOMBARDMENT; GIBBERELLIN CONJUGATE; PICEA-SITCHENSIS, SITKA SPRUCE
Publicerad i
Phytochemical Analysis
1992, volym: 3, nummer: 1, sidor: 32-37
Utgivare: JOHN WILEY & SONS LTD
SLU författare
UKÄ forskningsämne
Analytisk kemi
Publikationens identifierare
- DOI: https://doi.org/10.1002/pca.2800030106
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/42948