Analysis of Acid-Soluble Glycogen in Pork Extracts of Two PRKAG3 Genotypes by H-1 Liquid-State NMR Spectroscopy and Biochemical Methods

Sammanfattning

Meat extracts with acid-soluble glycogen (macroglycogen) from M. longissmus dorsi of carriers and noncarriers of the PRKAG3 mutation (RN- and rn(+) genotype) were analyzed by both H-1 liquid-state NMR spectroscopy and a biochemical method. The H-1 NMR analysis revealed that shorter polymers (dimers, trimers, etc.) of alpha-1,4-linked glucose were generated 24-48 h post-mortem. This is not possible to elucidate with the biochemical method, by which only the total amount of hydrolyzed glucose residues is determined. The shorter polymers were primarily formed in carriers of the PRKAG3 mutation, suggesting different postmortem glycogen degradation mechanisms in the two genotypes.

Nyckelord

glycogen; H-1 nuclear magnetic resonance (H-1 NMR); enzymatic analysis; pork; RN-gene; PRKAG3 mutation

Publicerad i

Journal of Agricultural and Food Chemistry
2011, volym: 59, nummer: 22, sidor: 11895-11902
Utgivare: American Chemical Society

SLU författare

• Essen-Gustavsson, Birgitta

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Jensen Waern, Marianne

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Karlsson, Anders

  • Københavns Universitet

• Lindahl, Gunilla

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Livsmedelsvetenskap
Husdjursvetenskap
Klinisk vetenskap

Publikationens identifierare

• DOI: https://doi.org/10.1021/jf201822p

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/43199