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Sammanfattning

Atypical bovine pestiviruses are related antigenically and phylogenetically to bovine viral diarrhea viruses (BVDV-1 and BVDV-2), and may cause the same clinical manifestations in animals. Glycoprotein E-rns of an atypical bovine pestivirus Th/04_KhonKaen was produced in a baculovirus expression system and was purified by affinity chromatography. The recombinant E-rns protein was used as an antigen in a microsphere immunoassay for the detection of antibodies against BVDV-1 and atypical bovine pestivirus. The diagnostic performance of the new method was evaluated by testing a total of 596 serum samples, and the assay was compared with enzyme-linked immunosorbent assay (ELISA). Based on the negative/positive cut-off median fluorescence intensity (MFI) value of 2800, the microsphere immunoassay had a sensitivity of 100% and specificity of 100% compared to ELISA. The immunoassay was able to detect antibodies against both BVDV-1 and the atypical pestivirus. This novel microsphere immunoassay has the potential to be multiplexed for simultaneous detection of antibodies against different bovine pathogens in a high-throughput and economical way. (C) 2012 Elsevier B.V. All rights reserved.

Nyckelord

Atypical bovine pestivirus; BVDV; ELISA; E-rns; Immunoassay; Luminex

Publicerad i

Journal of Virological Methods
2012, volym: 185, nummer: 2, sidor: 193-198
Utgivare: ELSEVIER SCIENCE BV

SLU författare

UKÄ forskningsämne

Molekylärbiologi
Biokemi

Publikationens identifierare

  • DOI: https://doi.org/10.1016/j.jviromet.2012.06.017

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/44969