Bankefors, Johan
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Abstract
The metabolite profiles from muscles and livers of Atlantic salmon were investigated using high resolution nuclear magnetic resonance (HR-NMR) spectroscopy of aqueous extracts and magic angle spinning (MAS) NMR spectroscopy of intact tissues. Comparison of the data showed that most small metabolites present in the aqueous extracts were also identified in the Carr-Purcell-Meiboom-Gill (CPMG) MAS NMR spectra of the intact tissues. Not only the total omega-3 fatty acid content, but also the EPA and DHA content, in the muscle and liver tissues could be calculated directly from the diffusion-edited MAS NMR spectra without the need for lipophilic extraction. The effect of replacing a normal fish meal during a period of 3 months with a diet containing 20% zygomycete was also investigated by multivariate analysis of the NMR spectra. Principal component analysis (PCA) was used to study the small metabolites distribution in the aqueous extracts of liver and muscles, and revealed that the feed containing zygomycete could have an influence on the metabolites profiles of juvenile Atlantic salmon. (C) 2011 Elsevier Ltd. All rights reserved.
Keywords
Fish meal replacement; Fatty acids; Metabolites; Muscle; Liver; Proton-NMR; Zygomycete
Published in
Food Chemistry
2011, volume: 129, number: 4, pages: 1397-1405
Publisher: ELSEVIER SCI LTD
SLU Authors
Schlechtriem, Christian
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Pickova, Jana
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Brännäs, Eva
- Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences
- Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences
Kiessling, Anders
- Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences
- Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences
Sandström, Corine
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Global goals (SDG)
UKÄ Subject classification
Food Science
Publication identifier
- DOI: https://doi.org/10.1016/j.foodchem.2011.05.081
Permanent link to this page (URI)
https://res.slu.se/id/publ/46476