Expression, purification, crystallization and preliminary X-ray diffraction studies of the cmcl component of Streptomyces clavuligerus 7 alpha-cephem-methoxylase

Sammanfattning

Cephamycins are broad-spectrum beta-lactam antibiotics that show resistance to certain forms of beta-lactamases. They differ from cephalosporins by the presence of a methoxyl group at the C-7alpha position. The gene products of cmcI and cmcJ are believed to control 7alpha-methoxylation of cephalosporins through successive steps of hydroxylation and methylation. Here, the expression, purification, crystallization and initial data-collection statistics of the 236-amino-acid protein product of cmcI from Streptomyces clavuligerus is reported. The crystals belong to space group P2(1), with unit-cell parameters a = 93.6, b = 182.6, c = 103.2 Angstrom, beta = 91.05degrees. Diffraction data were collected to 2.5 Angstrom

Publicerad i

Acta Crystallographica Section D: Biological Crystallography
2004, volym: 60, sidor: 1618-1621
Utgivare: BLACKWELL MUNKSGAARD

SLU författare

• Lester, Diane

  • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet
    

• Andersson, Inger

  • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet
    

• Öster, Linda

  • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet
    

Publikationens identifierare

• DOI: https://doi.org/10.1107/S090744490401666X

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/4781