Studies on the interactions between glycosylated beta(3)-peptides and the lectin Vicia villosa by saturation transfer difference NMR spectroscopy

Sammanfattning

Saturation transfer difference (STD) NMR spectroscopy was used to study the interaction of the lectin Vicia villosa (VVLB4) with alpha-D-GalNAc glycosylated beta(3)-peptides. The data were compared to those obtained with the monosaccharides D-Gal, D-GalNAc, and D-Glc as well as with those obtained with the Tn antigen alpha-glycopeptide (D-GalNAc-alpha-O-Ser/Thr), molecule naturally recognized by V. villosa. Evidence that the lectin also recognizes glycosylated beta(3)-peptides and has close contact with both the sugar and amino acid moieties was obtained. (C) 2009 Elsevier Ltd. All rights reserved.

Nyckelord

STD NMR; Glycosylated beta(3)-peptides; Tn antigen; Lectin Vicia villosa (isolectin B-4)

Publicerad i

Carbohydrate Research
2009, volym: 344, nummer: 18, sidor: 2577-2580
Utgivare: ELSEVIER SCI LTD

SLU författare

• Sandström, Corine

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Organisk kemi
Biokemi
Molekylärbiologi

Publikationens identifierare

• DOI: https://doi.org/10.1016/j.carres.2009.06.040

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/48887