Andersson, Lars
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
Determination of the absolute configuration of the 1-carboxyethyl substituent on a monosaccharide by circular dichroism measurements was found to be a sensitive and simple method. It relies on comparison of the spectrum of a 1-carboxyethyl substituted sugar or sugar derivative with the spectra of (R)- and (S)-lactic acid in the region 200-260 nm in which the (R)- and (S)-configuration give negative and positive Deltaepsilon, respectively. The oligo- or poly-saccharide containing a 1-carboxyethyl substituted sugar is hydrolyzed to monomers and the 1-carboxyethyl substituted sugar isolated by chromatography. The CD spectrum obtained for the 1-carboxyethyl substituted sugar in water solution at pH 2 is then compared with spectra of (R)- and (S)-lactic acid. The sign for the absorption and a maximum of comparable intensity and appearance around 210 nm, identify the stereochemistry. (C) 2002 Elsevier Science Ltd. All rights reserved.
Nyckelord
1-carboxyethyl substituent; circular dichroism; absolute configuration
Publicerad i
Carbohydrate Research
2003, volym: 338, nummer: 1, sidor: 85-93
Utgivare: ELSEVIER SCI LTD
SLU författare
Kenne, Lennart
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
- Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
Publikationens identifierare
- DOI: https://doi.org/10.1016/S0008-6215(02)00352-X
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/521