The unique structure of complete lipopolysaccharide isolated from semi-rough Plesiomonas shigelloides O37 ( strain CNCTC 39/89) containing (2S)-O-(4-oxopentanoic acid)-alpha-D-Glcp (alpha-D-Lenose)

Sammanfattning

in which alpha-D-Lenp is (2S)-O-(4-oxopentanoic acid)-alpha-D-Glcp residue which has not been found in nature. The absolute configuration of oxopentanoic acid moiety in alpha-D-Lenose residue was determined by NOESY experiment combined with molecular modeling (MM2 force field). The decasaccharide core is substituted at C-4 of the beta-D-Glcp residue with a single pentasaccharide unit. Lipid A is built of a beta-D-GlcpN4P-(1 -> 6)-alpha-D-GlcpN1P disaccharide asymmetrically substituted with fatty acids. It was concluded that the core oligosaccharide and the lipid A are identical with those in P. shigelloides CNCTC 113/ 92 Niedziela et al. (2002)(9) and Lukasiewicz et al. (2006).(10) (C) 2013 Published by Elsevier Ltd.

Nyckelord

Semi-rough lipopolysaccharide; Oxopentanoic acid; NMR; MALDI-TOF MS; MM2 force field; Plesiomonas shigelloides

Publicerad i

Carbohydrate Research
2013, volym: 378, sidor: 98-107
Utgivare: ELSEVIER SCI LTD

SLU författare

• Kenne, Lennart

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Strukturbiologi

Publikationens identifierare

• DOI: https://doi.org/10.1016/j.carres.2013.04.015

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/53315