Munir, Muhammad
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
- Sveriges Veterinärmedicinska Anstalt (SVA)
Sammanfattning
Equine arteritis virus (EAV) causes a respiratory and reproductive disease in horses, equine viral arteritis. Though cell death in infection with EAV is considered to occur by apoptosis, the underlying molecular mechanism has not been extensively elucidated. We investigated the expression of mRNA of proapoptotic and caspase genes during EAV infection in BHK21 cells, a well-established cell type for EAV replication. Using a SYBR Green real-time PCR, mRNA of p53, Bax, caspase 3 and caspase 9 were found up-regulated in a time dependent manner in EAV infected cells. Western blot analysis for caspase 3 and caspase 9 showed expression of cleaved forms of these proteins during EAV infection. In addition, a luminescence-based cell assay for caspase 3/7 activation as a hallmark in apoptosis confirmed apoptotic cell death. The findings demonstrate that cell death in EAV infected BHK21 cells results from apoptosis mediated through the intrinsic signalling pathway. (c) 2012 Elsevier B.V. All rights reserved.
Nyckelord
Equine arteritis virus; Apoptosis; Caspase 9; 3/7; p53; Bax; Real-time PCR
Publicerad i
Virus Research
2013, volym: 171, nummer: 1, sidor: 222-226
Utgivare: ELSEVIER SCIENCE BV
SLU författare
UKÄ forskningsämne
Annan veterinärmedicin
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.virusres.2012.10.004
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/53846