Sequencing methods have improved rapidly since the first versions of the Sanger techniques, facilitating the development of very powerful tools for detecting and identifying various pathogens, such as viruses, bacteria and other microbes. The ongoing development of high-throughput sequencing (HTS; also known as next-generation sequencing) technologies has resulted in a dramatic reduction in DNA sequencing costs, making the technology more accessible to the average laboratory. In this White Paper of the World Organisation for Animal Health (0IE) Collaborating Centre for the Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine (Uppsala, Sweden), several approaches and examples of HTS are summarised, and their diagnostic applicability is briefly discussed. Selected future aspects of HTS are outlined, including the need for bioinformatic resources, with a focus on improving the diagnosis and control of infectious diseases in veterinary medicine.
Aetiology; Detection of viruses and bacteria; Diagnosis; Emerging viruses and bacteria; High-throughput sequencing; Metagenomics; Next-generation sequencing
Revue Scientifique et Technique- Office International des Epizooties
2013, volym: 32, nummer: 3, sidor: 893-915
Utgivare: OFFICE INT EPIZOOTIES
Annan veterinärmedicin
https://res.slu.se/id/publ/55194