Fatty acids and gene expression responses to bioactive compounds in Atlantic salmon (Salmo salar L.) hepatocytes

Sammanfattning

OBJECTIVES: Effects of bioactive compounds on expression of genes involved in lipid metabolism and fatty acid composition were investigated in Atlantic salmon (Salmo salar L.) primary hepatocytes.  METHODS: Five treatments were investigated: I) genistein 0.005 mM, II) genistein 0.025 mM, III) lipoic acid 0.2 mM, IV) sesamin/episesamin 0.05 mM, V) sesamin 0.05 mM and compared to controls. The relative expression of genes involved in lipid homeostasis was analysed after 12h and 48h.  RESULTS: Incubation with lipoic acid, sesamin and episesamin/sesamin for 48h had significant effect on all analysed genes involved in lipid uptake, β-oxidation, elongation and desaturation, some effects were detected on the expression of peroxisome prolifertor-activated receptor (PPARs). Also effects on the fatty acid composition were found.  CONCLUSION: The strongest effect of bioactive copounds on hepatocyte gene expression was detected after 48 hours.

Nyckelord

beta-oxidation; desaturation; elongation; episesamin; lipoic acid; genistein

Publicerad i

Neuro Endocrinology Letters
2011, volym: 32 (Suppl. 2), sidor: 41-50
Utgivare: MAGHIRA & MAAS PUBLICATIONS

SLU författare

• Schiller Vestergren, Anna

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Trattner, Sofia

  • Institutionen för vilt, fisk och miljö, Sveriges lantbruksuniversitet
    
  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Mraz, Jan

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    
  • Jihočeská univerzita v Českých Budějovicích

• Pickova, Jana

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    
  • Jihočeská univerzita v Českých Budějovicích

UKÄ forskningsämne

Livsmedelsvetenskap

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/57113