Expression of four canine leukocyte adhesion factors in fresh and stored whole blood samples evaluated using a no-lyse, no-wash method

Sammanfattning

Expression of four leukocyte adhesion factors on canine leukocytes was studied by flow cytometry using a no-lyse, no-wash method. The effect of fixation and storage for up to 14 days in 1% paraformaldehyde on labelled samples and within assay variation was evaluated. Monoclonal antibodies directed to monocyte marker CD14, and to adhesion molecules CD11 a, CD18,CD32 and CD49d were used. Cell surface marker, cell population, time, and the interactions between time and cell marker significantly affected expression of cell adhesion factors. For CD18, there was a significant difference in mean fluorescence intensity (MFI) between fresh and stored samples (P

Nyckelord

Flow cytometry; Leukocyte; Cell surface; Activation; Inflammation

Publicerad i

Veterinary Immunology and Immunopathology
2011, volym: 139, nummer: 139, sidor: 271-276
Utgivare: ELSEVIER SCIENCE BV

SLU författare

• Ström Holst, Bodil

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Hagberg Gustavsson, Malin

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Lilliehöök, Inger

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Johannisson, Anders

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Klinisk vetenskap

Publikationens identifierare

• DOI: https://doi.org/10.1016/j.vetimm.2010.09.011

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/57197