Genetic diversity of root nodule bacteria nodulating Lotus corniculatus and Anthyllis vulneraria in Sweden

Ampomah, Osei Yaw; Huss-Danell, Kerstin

doi:10.1016/j.syapm.2011.01.006

Sammanfattning

Very little is known about the genetic diversity and phylogeny of rhizobia nodulating Lotus species in northern temperate regions. We have therefore studied the genetic diversity among a total of 61 root nodule bacteria isolated from Lotus corniculatus and Anthyllis vulneraria from different geographic sites and habitats in Sweden by restriction fragment length polymorphism (RFLP) of the internal transcribed spacer between their 16S rRNA and 23S rRNA (IGS) region. A high diversity consisting of 26 IGS types from 54 L. corniculatus isolates and five IGS types from seven A. vulneraria isolates was found. The 16S rRNA sequences and phylogeny of representatives of the different IGS types showed four interesting exceptions from the majority of the isolates belonging to the genus Mesorhizobium: Two isolates were both found to be closely related to Rhodococcus spp., and two other isolates showed close relationship with Geobacillus spp. and Paenibacillus spp., respectively. The nodA sequences and phylogeny showed that all the isolates, including those not belonging to the traditional rhizobia genera, harbored nodA sequences which were typical of Mesorhizobium loti. Generally, the 165 rRNA and nodA phylogenetic trees were not congruent in that isolates with similar 165 rRNA sequences were associated with isolates harboring different nodA sequences. All the isolates were confirmed to nodulate L. corniculatus in an inoculation test. This is the first report of members of these non-rhizobia genera being able to nodulate legumes, and we suggest that they may have acquired their nodulating properties through lateral gene transfer. (C) 2011 Elsevier GmbH. All rights reserved.

Nyckelord

Genetic diversity; Lotus corniculatus; Rhizobia; Phylogeny

Publicerad i

Systematic and Applied Microbiology
2011, volym: 34, nummer: 4, sidor: 267-275
Utgivare: ELSEVIER GMBH, URBAN & FISCHER VERLAG

SLU författare

Ampomah, Osei Yaw
- Institutionen för norrländsk jordbruksvetenskap, Sveriges lantbruksuniversitet
Huss-Danell, Kerstin
- Institutionen för norrländsk jordbruksvetenskap, Sveriges lantbruksuniversitet

UKÄ forskningsämne

Mikrobiologi

Publikationens identifierare

DOI: https://doi.org/10.1016/j.syapm.2011.01.006

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/57383