Wallgren, Margareta
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
- Quality Genetics
Sammanfattning
Aliquots of ejaculates were spiked with PCV2 and processed by a double processing technique, consisting of Single Layer Centrifugation on Androcoll (TM)-P followed by a "swim-up" procedure. Samples were collected from the resulting fractions during the selection process and analyzed for the presence of infectious PCV2. Virus titres were determined by performing a 50% tissue culture infective dose assay (TCID(50)) by end point dilution and with the use of an indirect peroxidise monolayer assay technique. With an initial infectious virus titre of 3.25-3.82 (TCID(50))/50 mu L the two-step sperm selection method eliminated 2.92 +/- 0.23 logs of infectious PCV2, corresponding to more than 99% reduction. Sperm quality was not affected by the selection procedure. (C) 2011 Elsevier B.V. All rights reserved.
Nyckelord
Porcine circovirus; Semen; Viral removal; Colloid centrifugation; Swim-up
Publicerad i
Animal Reproduction Science
2011, volym: 126, nummer: 1-2, sidor: 108-114
Utgivare: ELSEVIER SCIENCE BV
SLU författare
Morrell, Jane
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Husdjursvetenskap
Veterinärmedicin
Jordbruksvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.anireprosci.2011.04.014
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/58358