Xia, Hongyan
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes. (C) 2010 Elsevier B.V. All rights reserved.
Nyckelord
Luminex; Microsphere immunoassay; BVDV
Publicerad i
Journal of Virological Methods
2010, volym: 168, nummer: 1-2, sidor: 18-21
Utgivare: ELSEVIER SCIENCE BV
SLU författare
Belak, Sandor
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Husdjursvetenskap
Veterinärmedicin
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.jviromet.2010.04.009
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/60460