Kvarnheden, Anders
- Institutionen för växtbiologi, Sveriges lantbruksuniversitet
Sammanfattning
Background: Tomato yellow leaf curl virus (TYLCV) is a member of the family Geminiviridae, genus Begomovirus. To test the infectivity of TYLCV in tomato plants, an improved protocol for inoculation of in vitro-cultured tomato plants was developed.Results: A TYLCV isolate was cloned, sequenced and used to construct a 1.8-mer infectious clone. Three weeks old microshoots of TYLCV-susceptible tomato plants were inoculated with Agrobacterium tumefaciens harboring the infectious clone for the TYLCV isolate. After two weeks, the TYLCV symptoms started to appear on the in vitro-inoculated plants and the symptoms became more severe and pronounced eight weeks post-inoculation. The method was used efficiently to uncover the resistance mechanism against TYLCV in Solanum habrochaites accession LA 1777, a wild tomato known for its high resistance to whitefly and TYLCV.Conclusions: The reported in vitro-inoculation method can be used to screen tomato genotypes for their responses to TYLCV under controlled conditions and it will be a useful tool for better understanding of the TYLCV biology in tomato plants.
Publicerad i
Virology Journal
2010, volym: 7, artikelnummer: 84
Utgivare: BIOMED CENTRAL LTD
SLU författare
UKÄ forskningsämne
Jordbruksvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1186/1743-422X-7-84
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/60809