Aga, Esayas
- Institutionen för växtvetenskap, Sveriges lantbruksuniversitet
Sammanfattning
Genetic diversity in forest coffee (Coffea arabica L.) was estimated using inverse sequence-tagged repeat (ISTR) markers. One hundred ninety two samples representing 16 populations of C. arabica from four regions of Ethiopia were analyzed with 12 pairs of forward and backward ISTR primer combinations. A total of 144 reproducible bands were generated out of which 37 (25%) were polymorphic and scored as present (1) or absent (0) data matrix. This data was used to compute Jaccard coefficient to estimate genetic variability among all possible pairs of samples. The proportion of polymorphic bands within populations ranged from 19% for Bale-3, to 54% for Walega-2 populations. Un-weighted pair group method with arithmetic mean (UPGMA) based cluster analysis generated two clusters at 56% similarity value. The samples were clustered on the basis of their geographical origin, which could be attributed to a few region specific banding patterns detected. However, within regions most of the samples failed to cluster on the basis of their respective populations, which may be due to the presence of substantial gene flow between local populations in the form of seedlings carried out by farmers. The results may provide information to develop strategies for in situ conservation.
Nyckelord
Coffea arabica; conservation; Ethiopia; forest coffee; genetic diversity; ISTR
Publicerad i
Genetic Resources and Crop Evolution
2006, volym: 53, nummer: 4, sidor: 721-728
Utgivare: SPRINGER
SLU författare
Bryngelsson, Tomas
- Institutionen för växtvetenskap, Sveriges lantbruksuniversitet
- Institutionen för växtvetenskap, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Trädgårdsvetenskap/hortikultur
Publikationens identifierare
- DOI: https://doi.org/10.1007/s10722-004-5729-5
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/6388