Granberg, Fredrik
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Abstract
Metagenomic approaches have become invaluable for culture-independent and sequence-independent detection and characterization of disease-associated pathogens. Here, the sequential steps from sampling to verification of results are described for a metagenomic-based approach to detect potential pathogens in honeybees. The pre-sequencing steps are given in detail, but due to the rapid development of sequencing technologies, all platform-specific procedures, as well as subsequent bioinformatics analysis, are more generally described. It should also be noted that this approach could, with minor modifications, be adapted for other organisms and sample matrices.
Keywords
Virus; metagenomics
Published in
Methods in Molecular Biology
2015, volume: 1247, number: 1247, pages: 491-511
Title: Veterinary infection biology: molecular diagnostics and high-throughput strategies Methods in Molecular Biology
Publisher: Springer New York
SLU Authors
Karlsson Lindsjö, Oskar
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Belak, Sandor
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Associated SLU-program
Future Animal Health and Welfare (until Jan 2017)
UKÄ Subject classification
Microbiology in the medical area
Publication identifier
- DOI: https://doi.org/10.1007/978-1-4939-2004-4_33
- ISBN: 978-1-4939-2004-4
Permanent link to this page (URI)
https://res.slu.se/id/publ/65802