Granberg, Fredrik
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
Metagenomic approaches have become invaluable for culture-independent and sequence-independent detection and characterization of disease-associated pathogens. Here, the sequential steps from sampling to verification of results are described for a metagenomic-based approach to detect potential pathogens in honeybees. The pre-sequencing steps are given in detail, but due to the rapid development of sequencing technologies, all platform-specific procedures, as well as subsequent bioinformatics analysis, are more generally described. It should also be noted that this approach could, with minor modifications, be adapted for other organisms and sample matrices.
Nyckelord
Virus; metagenomics
Publicerad i
Methods in Molecular Biology
2015, volym: 1247, nummer: 1247, sidor: 491-511
Titel: Veterinary infection biology: molecular diagnostics and high-throughput strategies Methods in Molecular Biology
Utgivare: Springer New York
SLU författare
Karlsson Lindsjö, Oskar
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
Belak, Sandor
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
Associerade SLU-program
Framtidens djurhälsa och djurvälfärd (tom Jan 2017)
UKÄ forskningsämne
Mikrobiologi inom det medicinska området
Publikationens identifierare
- DOI: https://doi.org/10.1007/978-1-4939-2004-4_33
- ISBN: 978-1-4939-2004-4
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/65802