Orozovic, Kanita
- Institutionen för växtbiologi, Sveriges lantbruksuniversitet
Sammanfattning
Resistance to neuraminidase inhibitors (NAIs) is a growing problem in battle against influenza A virus. However, little is known about the resistance of viruses isolated from dabbling ducks, the natural reservoir of the influenza virus. To our knowledge, no low-pathogenic avian influenza (LPAI) virus resistant to NAIs has been detected. The aim of this study was to investigate mallard isolates of influenza A virus previously identified to carry oseltamivir carboxylate (OC) or zanamivir (ZA) resistance-related mutations. In this work, 21 viruses belonging to the N1, N3, N6 and N9 subtypes were analyzed using a colorimetric NA inhibition assay. The results of assay showed no NAIs-resistant phenotype for any of the viruses. The R118K mutation was the most recurrent, as it was observed in all subtypes except for N6. IC50 values confirmed the differences in sensitivity to OC or ZA observed in the N1 and N2 groups of NAs. Furthermore, both wild types (WTs) in the N6 and one WT in the N9 subtype were less sensitive to ZA than were genotypically related mutants with R152K and R118K change in the respective subtypes. This may indicate that these and probably even other NAIs resistance-related mutations found in our virus collection were not induced by NAIs residuals in the environment and that the impact of such mutations in an avian influenza could be dependent on subtype, strain and host species.
Publicerad i
PLoS ONE
2014, volym: 9, nummer: 2, artikelnummer: e89306
Utgivare: PUBLIC LIBRARY SCIENCE
SLU författare
Globala målen (SDG)
SDG3 God hälsa och välbefinnande
UKÄ forskningsämne
Farmakologi och toxikologi
Publikationens identifierare
- DOI: https://doi.org/10.1371/journal.pone.0089306
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/66871