Linne, Tommy
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
The DNA assisted solid-phase proximity ligation assay (SP-PLA) provides a unique opportunity to specifically detect prion protein (PrP) aggregates by investigating the collocation of 3 or more copies of the specific protein. We have developed an SP-PLA that can detect PrP aggregates in brain homogenates from infected hamsters even after a 10(7)-fold dilution. In contrast, brain homogenate from uninfected animals did not generate a detectable signal at 100-fold higher concentration. Using either of the 2 monoclonal anti-PrP antibodies, 3F4 and 6H4, we successfully detected low concentrations of aggregated PrP. The presented results provide a proof of concept that this method might be an interesting tool in the development of diagnostic approaches of prion diseases.
Nyckelord
diagnosis; monoclonal antibody; prion protein; proximity ligation assay; 263K
Publicerad i
Prion
2014, volym: 8, nummer: 3, sidor: 261-265
Utgivare: LANDES BIOSCIENCE
SLU författare
UKÄ forskningsämne
Biokemi
Molekylärbiologi
Publikationens identifierare
- DOI: https://doi.org/10.4161/pri.32231
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/67818