Borisova, Anna
- National Research Centre - Kurchatov Institute
- The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences
Abstract
In the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters k(cat), K-m and product K-I for the hydrolysis of a p-nitrophenyl glycoside by an exo-acting glycoside hydrolase can be estimated in a single experiment. Its applicability was demonstrated for three retaining exo-glycoside hydrolases, beta-xylosidase from Aspergillus awamori, beta-galactosidase from Penicillium sp. and alpha-galactosidase from Thermotoga maritima (TmGalA). During the analysis of the reaction course catalyzed by the TmGalA enzyme we had observed that a non-enzymatic process, mutarotation of the liberated alpha-D-galactose, affected the reaction significantly. (C) 2015 Elsevier Ltd. All rights reserved.
Keywords
Integrated kinetics; Retaining glycoside hydrolase; Mutarotation
Published in
Carbohydrate Research
2015, volume: 412, pages: 43-49
Publisher: ELSEVIER SCI LTD
SLU Authors
Sandgren, Mats
- The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences
- The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Organic Chemistry
Biophysics
Publication identifier
- DOI: https://doi.org/10.1016/j.carres.2015.03.021
Permanent link to this page (URI)
https://res.slu.se/id/publ/68450