Borisova, Anna
- Institutionen för kemi och bioteknologi, Sveriges lantbruksuniversitet
- National Research Centre - Kurchatov Institute
Sammanfattning
In the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters k(cat), K-m and product K-I for the hydrolysis of a p-nitrophenyl glycoside by an exo-acting glycoside hydrolase can be estimated in a single experiment. Its applicability was demonstrated for three retaining exo-glycoside hydrolases, beta-xylosidase from Aspergillus awamori, beta-galactosidase from Penicillium sp. and alpha-galactosidase from Thermotoga maritima (TmGalA). During the analysis of the reaction course catalyzed by the TmGalA enzyme we had observed that a non-enzymatic process, mutarotation of the liberated alpha-D-galactose, affected the reaction significantly. (C) 2015 Elsevier Ltd. All rights reserved.
Nyckelord
Integrated kinetics; Retaining glycoside hydrolase; Mutarotation
Publicerad i
Carbohydrate Research
2015, volym: 412, sidor: 43-49
Utgivare: ELSEVIER SCI LTD
SLU författare
Sandgren, Mats
- Institutionen för kemi och bioteknologi, Sveriges lantbruksuniversitet
- Institutionen för kemi och bioteknologi, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Organisk kemi
Biofysik
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.carres.2015.03.021
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/68450