Rising, Anna
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
- Karolinska institutet
Sammanfattning
There is a great need for defined cell culture systems that allow expansion of human pluripotent stem cells (hPSCs) and subsequent controlled differentiation, ideally in an implantable three-dimensional (3D) matrix. Spider silk appears to be an ideal biomaterial, since it is strong, extendible, and has favorable properties when implanted in living tissues. Spiders are difficult to house and therefor methods for recombinant production of spider silk are warranted. We have developed a method for production of recombinant spider silk fibers, films and foams that are used for the design of defined and xeno-free cell culture matrices. The matrices enable long-term expansion of multiple hPSC lines and subsequent differentiation into all three germ layers in 3D. This hPSC culture method provides robust, defined, easily produced and flexible culture environments for hPSCs (1). These matrices are promising but to realize their full potential, we need to spin continuous fibers in a reproducible way. Spider silk fibers are produced from soluble spidroins under ambient conditions. The spidroins are large and highly repetitive in sequence but capped by non-repetitive N- and C-terminal domains (NT and CT). In the gland, a pH gradient, that goes from 7.6 to
Publicerad i
Utgivare: MRS (Materials Research Society)
Konferens
2015 MRS Spring Meeting. San Francisco, California. April 6-10
SLU författare
Andersson, Marlene
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
Wu, Siqin
- Karolinska institutet
Johansson, Jan
- Karolinska institutet
UKÄ forskningsämne
Cell- och molekylärbiologi
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/68656