NMR Study of the O-Specific Polysaccharide and the Core Oligosaccharide from the Lipopolysaccharide Produced by Plesiomonas shigelloides O24:H8 (Strain CNCTC 92/89)

Lundqvist, Lena; Kaszowska, Marta; Sandström, Corine

doi:10.3390/molecules20045729

Abstract

The structures of the O-specific polysacccharide and core oligosaccharide of the lipopolysaccharide from Plesiomonas shigelloides O24:H8, strain CNCTC 92/89, have been investigated by NMR spectroscopy and ESI mass spectrometry. The O-specific polysaccharide was found to be composed of a tetrasaccharide repeating unit consisting of [3)--FucpNAc-(13)--GalpNAcA-(13)--QuipNAc-(1] and of -RhapNAc (14) linked to the GalpNAcA residue. An identical structure has been reported for the capsular polysaccharide of the clinical isolate of Vibrio vulnificus strain BO62316 [1]. The core oligosaccharide was composed of a decasaccharide which structure is identical with these in P. shigelloides serotype O54 [2] and serotype O37 [3].

Keywords

lipopolysaccharide; Plesiomonas shigelloides; NMR; ESI MS; structural analysis

Published in

Molecules
2015, volume: 20, number: 4, pages: 5729-5739
Publisher: MDPI AG

SLU Authors

Lundqvist, Lena
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Sandström, Corine
- The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences

UKÄ Subject classification

Analytical Chemistry

Publication identifier

DOI: https://doi.org/10.3390/molecules20045729

Permanent link to this page (URI)

https://res.slu.se/id/publ/69714