NMR Study of the O-Specific Polysaccharide and the Core Oligosaccharide from the Lipopolysaccharide Produced by Plesiomonas shigelloides O24:H8 (Strain CNCTC 92/89)

Sammanfattning

The structures of the O-specific polysacccharide and core oligosaccharide of the lipopolysaccharide from Plesiomonas shigelloides O24:H8, strain CNCTC 92/89, have been investigated by NMR spectroscopy and ESI mass spectrometry. The O-specific polysaccharide was found to be composed of a tetrasaccharide repeating unit consisting of [3)--FucpNAc-(13)--GalpNAcA-(13)--QuipNAc-(1] and of -RhapNAc (14) linked to the GalpNAcA residue. An identical structure has been reported for the capsular polysaccharide of the clinical isolate of Vibrio vulnificus strain BO62316 [1]. The core oligosaccharide was composed of a decasaccharide which structure is identical with these in P. shigelloides serotype O54 [2] and serotype O37 [3].

Nyckelord

lipopolysaccharide; Plesiomonas shigelloides; NMR; ESI MS; structural analysis

Publicerad i

Molecules
2015, volym: 20, nummer: 4, sidor: 5729-5739
Utgivare: MDPI AG

SLU författare

• Lundqvist, Lena

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Sandström, Corine

  • Institutionen för kemi och bioteknologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Analytisk kemi

Publikationens identifierare

• DOI: https://doi.org/10.3390/molecules20045729

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/69714