Morrell, Jane
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
To date, the only repeatable method to select spermatozoa for chromosomal sex is the Beltsville sorting technology using flow cytometry. Improvement of this technology in the equine species requires increasing awareness of the modifications that the sorting procedure induces on sperm intactness. Oxidative stress is regarded as the major damaging phenomenon, and increasing evidence regards handling of spermatozoa - including sex sorting - as basic ground for oxidative damage. The aim of this study was to disclose whether the flow cytometric sorting procedure increases the production of reactive oxygen species (ROS), and to identify if ROS production relates to DNA damage in sorted spermatozoa using specific flow cytometry-based assays. After sorting, oxidative stress increased from 26% to 33% in pre- and post-incubation controls, to 46% after sex sorting (p < 0.05). Proportions of DNA fragmentation index post-sorting were approximately 10% higher (31.3%); an effect apparently conduced via oxidative DNA damage as revealed by the oxyDNA assay. The probable origin of this increased oxidative stress owes the removal of enough seminal plasma due to the unphysiological sperm extension, alongside a deleterious effect of high pressure on mitochondria during the sorting procedure.
Nyckelord
Reactive oxygen species; DNA fragmentation; OxyDNA Assay; seminal plasma
Publicerad i
Reproduction in Domestic Animals
2016, volym: 51, nummer: 1, sidor: 18-25
Utgivare: Wiley-Blackwell
SLU författare
UKÄ forskningsämne
Annan veterinärmedicin
Publikationens identifierare
- DOI: https://doi.org/10.1111/rda.12640
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/71499