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Sammanfattning

The study aimed at evaluating an effective sterilization-inoculation technique to facilitate silage research on the effect of forage microflora on fermentation variables. The sterilization effect of heating at 60 degrees C for 3h+103 degrees C for 15h was tested on samples of grass, grass-clover, white clover and maize, pre-dried at 60 degrees C to a dry-matter (DM) content >900gkg(-1). The ensilability of treated samples, reconstituted to original DM concentration (250-390gkg(-1)), was assessed by inoculation with microfloras extracted from the original samples. Microfloral inoculants were obtained by a combination of centrifugation (15500g for 40min) and filtration (045 and 022m pore sizes) of the supernatant. The sterilization treatment effectively sterilized the forage samples but decreased water soluble carbohydrates by 49% and N buffer solubility by 22% and increased the acid detergent insoluble N proportion of total N by 53% (P005). It is concluded that, despite causing chemical and physical alterations, the sterilization-inoculation technique evaluated could be a useful tool for future studies on the effects of microflora on ensiling results.

Nyckelord

inoculation; microflora; silage; sterilization

Publicerad i

Grass and Forage Science
2015, volym: 70, nummer: 4, sidor: 668-673
Utgivare: WILEY-BLACKWELL

SLU författare

UKÄ forskningsämne

Mikrobiologi

Publikationens identifierare

  • DOI: https://doi.org/10.1111/gfs.12153

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/72287