Wallerman, Ola
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
Background: ChIP-seq is the method of choice for genome-wide studies of protein-DNA interactions. We describe a new method for ChIP-seq sample preparation, termed lobChIP, where the library reactions are performed on crosslinked ChIP fragments captured on beads.Results: The lobChIP method was found both to reduce time and cost and to simplify the processing of many samples in parallel. lobChIP has an early incorporation of barcoded sequencing adaptors that minimizes the risk of sample cross-contamination and can lead to reduced amount of adaptor dimers in the sequencing libraries, while allowing for direct decross-linking and amplification of the sample.Conclusions: With results for histone modifications and transcription factors, we show that lobChIP performs equal to or better than standard protocols and that it makes it possible to go from cells to sequencing ready libraries within a single day.
Nyckelord
ChIP-seq; Chromatin immunoprecipitation; Illumina; NGS; Library preparation
Publicerad i
Epigenetics and Chromatin
2015, volym: 8, artikelnummer: 25
Utgivare: BIOMED CENTRAL LTD
SLU författare
UKÄ forskningsämne
Molekylärbiologi
Biokemi
Publikationens identifierare
- DOI: https://doi.org/10.1186/s13072-015-0017-5
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/75594