Isolation of Apoplast

Abstract

The apoplast can be described as the soluble fraction of the extracellular space of plant tissue, and it plays an important role in signaling, nutrient transport, and plant-pathogen interactions. In this protocol, we describe a method where leaves are infiltrated with phosphate buffer under vacuum. The apoplast can then be extracted by centrifugation and simultaneously collected in a protease inhibitor solution. Using this protocol, typically 3 mu g of apoplastic proteins can be obtained in a volume of 300 mu L from five potato leaflets, with minimal contamination by non-apoplastic proteins.

Keywords

Apoplast; Apoplastic protein; Secretome; Soluble fraction; Protease inhibitor; Phosphate buffer; Vacuum infiltration; Extracellular space

Published in

Methods in Molecular Biology
2017, volume: 1511, number: 1511, pages: 233-240
Title: Isolation of Plant Organelles and Structures
Publisher: Springer

SLU Authors

• Andreasson, Erik

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Abreha, Kibrom

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Resjö, Svante

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication identifier

• DOI: https://doi.org/10.1007/978-1-4939-6533-5_18
• ISBN: 978-1-4939-6531-1

Permanent link to this page (URI)

https://res.slu.se/id/publ/77554